{"id":1610,"date":"2021-08-09T16:47:57","date_gmt":"2021-08-09T16:47:57","guid":{"rendered":"http:\/\/wp.cov19longhaulfoundation.org\/?p=1610"},"modified":"2021-08-09T16:47:57","modified_gmt":"2021-08-09T16:47:57","slug":"rapid-generation-of-durable-b-cell-memory-to-sars-cov-2-spike-and-nucleocapsid-proteins-in-covid-19-and-convalescence","status":"publish","type":"post","link":"https:\/\/cov19longhaulfoundation.org\/?p=1610","title":{"rendered":"Rapid generation of durable B cell memory to SARS-CoV-2 spike and nucleocapsid proteins in COVID-19 and convalescence"},"content":{"rendered":"\n

Authors: Gemma E Hartley<\/a>\u00a01<\/a><\/sup>,\u00a0Emily S J Edwards<\/a>\u00a01<\/a><\/sup>,\u00a0Pei M Aui<\/a>\u00a01<\/a><\/sup>,\u00a0Nirupama Varese<\/a>\u00a01<\/a>\u00a02<\/a><\/sup>,\u00a0Stephanie Stojanovic<\/a>\u00a03<\/a><\/sup>,\u00a0James McMahon<\/a>\u00a04<\/a>\u00a05<\/a><\/sup>,\u00a0Anton Y Peleg<\/a>\u00a04<\/a>\u00a06<\/a><\/sup>,\u00a0Irene Boo<\/a>\u00a07<\/a><\/sup>,\u00a0Heidi E Drummer<\/a>\u00a07<\/a>\u00a08<\/a>\u00a09<\/a><\/sup>,\u00a0P Mark Hogarth<\/a>\u00a01<\/a>\u00a010<\/a>\u00a011<\/a><\/sup>,\u00a0Robyn E O’Hehir<\/a>\u00a01<\/a>\u00a02<\/a>\u00a03<\/a><\/sup>,\u00a0Menno C van Zelm<\/a>\u00a012<\/a>\u00a02<\/a><\/sup><\/p>\n\n\n\n

Abstract<\/h2>\n\n\n\n

Lasting immunity following SARS-CoV-2 infection is questioned because serum antibodies decline in convalescence. However, functional immunity is mediated by long-lived memory T and B (Bmem) cells. Therefore, we generated fluorescently-labeled tetramers of the spike receptor binding domain (RBD) and nucleocapsid protein (NCP) to determine the longevity and immunophenotype of SARS-CoV-2-specific Bmem cells in COVID-19 patients. A total of 36 blood samples were obtained from 25 COVID-19 patients between 4 and 242 days post-symptom onset including 11 paired samples. While serum IgG to RBD and NCP was identified in all patients, antibody levels began declining at 20 days post-symptom onset. RBD- and NCP-specific Bmem cells predominantly expressed IgM+<\/sup> or IgG1+<\/sup> and continued to rise until 150 days. RBD-specific IgG+<\/sup> Bmem were predominantly CD27+<\/sup>, and numbers significantly correlated with circulating follicular helper T cell numbers. Thus, the SARS-CoV-2 antibody response contracts in convalescence with persistence of RBD- and NCP-specific Bmem cells. Flow cytometric detection of SARS-CoV-2-specific Bmem cells enables detection of long-term immune memory following infection or vaccination for COVID-19.<\/p>\n\n\n\n

Copyright \u00a9 2020, American Association for the Advancement of Science.<\/p>\n\n\n\n

For More Information: https:\/\/pubmed.ncbi.nlm.nih.gov\/33443036\/<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Authors: Gemma E Hartley\u00a01,\u00a0Emily S J Edwards\u00a01,\u00a0Pei M Aui\u00a01,\u00a0Nirupama Varese\u00a01\u00a02,\u00a0Stephanie Stojanovic\u00a03,\u00a0James McMahon\u00a04\u00a05,\u00a0Anton Y Peleg\u00a04\u00a06,\u00a0Irene Boo\u00a07,\u00a0Heidi E Drummer\u00a07\u00a08\u00a09,\u00a0P Mark Hogarth\u00a01\u00a010\u00a011,\u00a0Robyn E O’Hehir\u00a01\u00a02\u00a03,\u00a0Menno C van Zelm\u00a012\u00a02 Abstract Lasting immunity following SARS-CoV-2 infection […]<\/p>\n","protected":false},"author":2,"featured_media":1611,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[22,982,101,836,964],"tags":[],"class_list":["post-1610","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-antibody","category-b-cell","category-covid-19","category-immune-system","category-t-cell"],"_links":{"self":[{"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=\/wp\/v2\/posts\/1610","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1610"}],"version-history":[{"count":0,"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=\/wp\/v2\/posts\/1610\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=\/wp\/v2\/media\/1611"}],"wp:attachment":[{"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1610"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1610"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1610"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}