{"id":2220,"date":"2021-08-16T15:30:08","date_gmt":"2021-08-16T15:30:08","guid":{"rendered":"http:\/\/wp.cov19longhaulfoundation.org\/?p=2220"},"modified":"2021-08-16T15:30:08","modified_gmt":"2021-08-16T15:30:08","slug":"the-oc43-human-coronavirus-envelope-protein-is-critical-for-infectious-virus-production-and-propagation-in-neuronal-cells-and-is-a-determinant-of-neurovirulence-and-cns-pathology","status":"publish","type":"post","link":"https:\/\/cov19longhaulfoundation.org\/?p=2220","title":{"rendered":"The OC43 human coronavirus envelope protein is critical for infectious virus production and propagation in neuronal cells and is a determinant of neurovirulence and CNS pathology"},"content":{"rendered":"\n<p>Authors:<a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0042682217304361#!\">Jenny K.Stodola<sup>1<\/sup>GuillaumeDubois<sup>1<\/sup>AlainLe CoupanecMarcDesforgesPierre J.Talbot<\/a><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Highlights<\/h2>\n\n\n\n<p id=\"sp0055\" style=\"font-size:24px\">Coronavirus structural envelope (E) protein specific motifs involved in protein-protein interaction or in homo-oligomeric ion channel formation are needed for optimal production of recombinant infectious virus.\u2022<\/p>\n\n\n\n<p id=\"p0010\" style=\"font-size:24px\">Fully functional E protein of HCoV-OC43 is crucial for viral propagation in the CNS and neurovirulence.\u2022<\/p>\n\n\n\n<p id=\"p0015\" style=\"font-size:24px\">Fully functional E protein of HCoV-OC43 is crucial for efficient viral propagation in the central nervous system and thereby for neurovirulence.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Abstract<\/h2>\n\n\n\n<p id=\"sp0050\" style=\"font-size:24px\">The OC43 strain of\u00a0<a href=\"https:\/\/www.sciencedirect.com\/topics\/medicine-and-dentistry\/coronaviridae\">human coronavirus<\/a>\u00a0(HCoV-OC43) is an ubiquitous respiratory tract pathogen possessing neurotropic capacities.\u00a0<a href=\"https:\/\/www.sciencedirect.com\/topics\/medicine-and-dentistry\/coronavirinae\">Coronavirus<\/a>\u00a0structural envelope (E) protein possesses specific motifs involved in\u00a0<a href=\"https:\/\/www.sciencedirect.com\/topics\/medicine-and-dentistry\/protein-protein-interaction\">protein-protein interaction<\/a>\u00a0or in homo-oligomeric ion channel formation, which are known to play various roles including in virion morphology\/assembly and in cell response to infection and\/or virulence. Making use of\u00a0<a href=\"https:\/\/www.sciencedirect.com\/topics\/medicine-and-dentistry\/virus-recombinant\">recombinant viruses<\/a>\u00a0either devoid of the E protein or harboring mutations either in putative transmembrane domain or PDZ-binding motif, we demonstrated that a fully functional HCoV-OC43 E protein is first needed for optimal production of recombinant\u00a0<a href=\"https:\/\/www.sciencedirect.com\/topics\/medicine-and-dentistry\/virus-infectivity\">infectious viruses<\/a>. Furthermore, HCoV-OC43 infection of human epithelial and\u00a0<a href=\"https:\/\/www.sciencedirect.com\/topics\/medicine-and-dentistry\/neuronal-cell-line\">neuronal cell lines<\/a>, of mixed murine primary cultures from the central nervous system and of mouse central nervous system showed that the E protein is critical for efficient and optimal virus replication and propagation, and thereby for neurovirulence.<\/p>\n\n\n\n<p class=\"has-large-font-size\">For More Information: <a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0042682217304361\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0042682217304361<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Authors:Jenny K.Stodola1GuillaumeDubois1AlainLe CoupanecMarcDesforgesPierre J.Talbot Highlights Coronavirus structural envelope (E) protein specific motifs involved in protein-protein interaction or in homo-oligomeric ion channel formation are needed for optimal production of recombinant infectious [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":2221,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[58,101,151,361,365,632,633],"tags":[],"class_list":["post-2220","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-brain","category-covid-19","category-epithelium","category-nerve-cell-injury","category-neurologic-effects","category-virus-propagation","category-virus-replication"],"_links":{"self":[{"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=\/wp\/v2\/posts\/2220","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2220"}],"version-history":[{"count":0,"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=\/wp\/v2\/posts\/2220\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=\/wp\/v2\/media\/2221"}],"wp:attachment":[{"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2220"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2220"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cov19longhaulfoundation.org\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2220"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}